Celsense Products - Cell Sense

 

 

Celsense Products - Cell Sense

Cellular therapeutic strategies often include using transplanted cells to reconstitute lesioned tissues or to stimulate an immune response. A challenge in the development and translation of these emerging therapies is effective tracking of cells post-transfer in both animal and human subjects. Cell Sense is a fluorocarbon-based emulsion engineered to safely and efficiently label cells ex vivo without the use of transfection reagents. Labeled cells are transferred into an animal or human subject enabling investigators to non–invasively track the administration and migration of therapeutic cells, quickly and quantitatively, using 19F magnetic resonance imaging (MRI) or nuclear magnetic resonance spectroscopy (i.e., NMR or MRS). The key advantage of 19F detection is that there is no background signal from the host’s tissues and only labeled cells are detected. Quantification of the 19F signal yields a fast, accurate marker of the number of transferred cells in regions of interest in vivo using MRI, or in intact, excised tissue samples using NMR. Importantly, Cell Sense labeling has no apparent impact on the viability, phenotype, or functional properties of cells. Cell Sense is currently manufactured in the United States under GMP conditions and is suitable for use in preclinical studies and clinical trials under an approved Investigational New Drug (IND) application.


Schematic of the Cell Sense cell tracking platform. Cells of interest are co-cultured ex vivo with the Cell Sense reagent, resulting in intracellular uptake. The labeled cells are then inoculated into a subject and imaged using MRI. Images of both the 19F (labeled cells) and 1H (background anatomy) are acquired in the same imaging session. A 19F/1H fusion image is constructed showing the regions containing labeled cells (red) in their anatomical context. Image fusion, visualization and cell quantification is performed using the Voxel Tracker™ software program. Alternatively, intact tissues samples excised from the subject are rapidly assayed using conventional liquid-state 19F NMR to determine cell number or cell density, thereby minimizing histological endpoints.

A dual–mode version of Cell Sense, detectable using either MRI/NMR or conventional fluorescence detection modalities (e.g., flow cytometry and optical microscopy), is available to simplify validation studies. Using the Voxel Tracker software package, investigators can visualize and quantify the number of labeled cells in user–defined regions directly from the in vivo MRI data.

Applications of the Cell Sense platform include cell tracking in immunotherapy or regenerative medicine, as well as diagnosis of inflammatory sites by monitoring selected populations of immune cells.

For more information about Cell Sense see:

Cell Sense: A Reagent to Non-Invasively Track the Delivery, Migration and Persistence of Cells Administered for Therapeutic and Diagnostic Purposes (PDF)